Which method is used to test for the presence of chlamydia and gonorrhea genes?

Which method is used to test for the presence of chlamydia and gonorrhea genes?

• A. Polymerase chain reaction (PCR)
• B. DNA probe test
• C. Western blot test
• D. Enzyme-linked immunosorbent assay (ELISA)

Answer: (A)

Detecting the pathogens at the genetic level is best accomplished by amplifying their DNA. PCR is a nucleic acid amplification method that targets DNA sequences unique to Chlamydia trachomatis and Neisseria gonorrhoeae. By repeatedly copying these target regions, even small amounts of bacterial DNA in a specimen become detectable, giving high sensitivity and specificity and allowing rapid, direct confirmation from clinical samples. Other approaches either detect proteins or use DNA hybridization without amplification, which are typically less sensitive or specific for identifying the presence of the organisms. Therefore, PCR is the most effective method for testing presence of chlamydia and gonorrhea genes.

Detecting the pathogens at the genetic level is best accomplished by amplifying their DNA. PCR is a nucleic acid amplification method that targets DNA sequences unique to Chlamydia trachomatis and Neisseria gonorrhoeae. By repeatedly copying these target regions, even small amounts of bacterial DNA in a specimen become detectable, giving high sensitivity and specificity and allowing rapid, direct confirmation from clinical samples. Other approaches either detect proteins or use DNA hybridization without amplification, which are typically less sensitive or specific for identifying the presence of the organisms. Therefore, PCR is the most effective method for testing presence of chlamydia and gonorrhea genes.